In addition to a neutral glycan, lipopolysaccharide extracts from the reference strain for Serratia marcescens serogroup O22 contain an acidic polymer which probably defines the serogroup and is of microcapsular origin. The polymer is doubly branched with a heptasaccharide repeating unit and a galac
Structural studies of an acidic galactomannan from the reference strain for Serratia marcescens serogroup O4
β Scribed by David Oxley; Stephen G. Wilkinson
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- English
- Weight
- 465 KB
- Volume
- 179
- Category
- Article
- ISSN
- 0008-6215
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β¦ Synopsis
An acidic, partially acetylated galactomannan has been isolated from the lipopolysaccharide of the reference strain (C.D.C. 864-57) for Serratia marcescens serogroup O4. From the results of methylation analysis, Smith degradations, and n.m.r. spectroscopic studies of the O-deacetylated polymer, it was concluded that the repeating unit has the structure shown, in which the acetal-linked pyruvic acid has the R configuration. The polymer is believed to confer O specificity on the organism, but not to constitute the side chain of the lipopolysaccharide. (formula; see text).
π SIMILAR VOLUMES
The lipopolysaccharide extract from the cell wall of the reference strain for Serratia marcescens serogroup O18 contained, in addition to a neutral glycan characterised previously, an acidic glycan. Acidity was contributed both by D-glucuronic acid and by 4-O-[(R)-1-carboxyethyl]-D-glucose (4-O-Lac-
Partially acetylated glucorhamnans have been isolated from the lipopolysaccharides of three strains of Serratia marcescens. The polymer from the reference strain (C.D.C. 864-57) for serogroup O4 has the disaccharide repeating-unit shown below, in which acetylation at position 2 of the rhamnosyl resi
The structure of the acidic polysaccharide from Serratia marcescens serogroup O1 has been investigated. NMR spectroscopy together with sugar and methylation analysis have been used as well as a uronic acid degradation. The polysaccharide consists of pentasaccharide repeating units having the followi
A partially acetylated acidic galactorhamnan has been isolated from the lipopolysaccharide of the 02 reference strain (C.D.C. 868-57) of Serratia marcescens. By means of n.m.r. spectroscopy and methylation analysis of the polymer and the products of Smith degradations, the repeating unit of the poly