Structure of a new N-acetylisomuramic acid-containing O-specific polysaccharide of Proteus penneri strains 19 and 35

✍ Scribed by Sof'ya N. Senchenkova; Alexander S. Shashkov; Yuriy A. Knirel; Nikolay K. Kochetkov; Krystyna Zych; Zygmunt Sidorczyk

• Publisher: Elsevier Science
• Year: 1996
• Tongue: English
• Weight: 398 KB
• Volume: 293
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(96)00192-9

✦ Synopsis

O-Specific polysaccharides, together with oligosaccharide products of their degradation, were isolated by GPC after mild acid delipidation of lipopolysaccharides of Proteus penneri strains 19 and 35. The pnlysaccharides had the same trisaccharide repeating unit containing one residue each of D-galactose, 2-acetamido-2-deoxy-D-glucose, and 2-acetamido-3-O-[(S)-l-carboxyethyl]-2deoxy-D-glucose (N-acetylisomuramic acid). On the basis of 1D and 2D IH and 13C NMR spectroscopy, including 2D correlation spectroscopy (COSY), rotating-frame NOE spectroscopy (ROESY), and H-detected heteronuclear I H,13C multiple-quantum coherence (HMQC), the following structure of the repeating unit was established:

The oligosaccharide products formed by cleavage of the glycosidic linkage of GlcNAc represent a chemical trisaccharide repeating unit of the polysaccharide and its oligomer homologs. The ease of hydrolysis of the polysaccharide is associated with the closeness of the glycosyl group and the * Corresponding author.