Structure of the O-specific polysaccharide of Proteus penneri 62 containing 2-acetamido-3-O-[(S)-1-carboxyethyl]-2-deoxy-d-glucose (N-acetylisomuramic acid)

✍ Scribed by Yuriy A. Knirel; Nikolay A. Paramonov; Evgeny V. Vinogradov; Alexander S. Shashkov; Nikolay K. Kochetkov; Zygmunt Sidorczyk; Anna Swierzko

• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 261 KB
• Volume: 235
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(92)80105-a

✦ Synopsis

Recently'**, the O-specific polysaccharides of two Proteus penner-i strains 14 and 16 have been structurally elucidated and found to contain N-linked I.-alanine, N-acetyl-o-alanyl groups, and (RI-3-hydroxybutyryl groups as non-sugar substituents. We report now the structure of the O-antigen of strain 62 of this new Proteus species, which includes a residue of @)-lactic acid.

The polysaccharide (PS-I) was obtained by mild acid degradation of the lipopolysaccharide, isolated from bacterial cells by the phenol-water procedure3. As judged by the 'H and 13C NMR spectra, PS-I lacked strict regularity, most probably due to the presence of OAc groups in a non-stoichiometric amount (a,, 2.13, 6, 21.6).

Treatment of PSI with 10% aqueous ammonia (60", 2 h) led to an O-deacetylated polysaccharide (PS-II), which had a trisaccharide repeating unit; there were signals for three anomeric protons at 6 4.