✦ LIBER ✦

Structure Investigation and Proton and Carbon-13 Assignments of Digitonin and Cholesterol using Multidimensional NMR Techniques

✍ Scribed by Petra Muhr; Werner Likussar; Manfred Schubert-Zsilavecz

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 395 KB
Volume: 34
Category: Article
ISSN: 0749-1581
DOI: 10.1002/(sici)1097-458x(199602)34:2<137::aid-omr841>3.0.co;2-q

No coin nor oath required. For personal study only.

✦ Synopsis

Digitonin, a spirostanolglycoside from the seeds of Digitalis purpurea L., is a highly haemolytically active saponin. This effect is considered to be based on complex formation between cholesterin and digitonin. Against the background of investigations concerning the interaction between glycosides and membrane components, and the determination of the structure of the digitonin-cholesterol complex, a detailed NMR spectroscopic investigation of the complex-forming compounds was carried out. Assignment of the 'H and 13C spectra of digitonin was made by using one-and two-dimensional NMR techniques (1D TOCSY, 2D TOCSY, HMQC and HMBC); 3D TOCSY-ROESY, 1D ROESY and HMBC experiments provided information about the sequence of the oligosaccharide chain and the position of glycosidations. The precise analysis of the COSY, HMQC und HMBC spectra made the assignment of all proton resonance signals of cholesterol possible for the first time.

📜 SIMILAR VOLUMES

Total Assignment of 1H and 13C NMR Chemi

Total Assignment of 1H and 13C NMR Chemical Shifts of a Natural Anthocyanidin, Apigeninidin, Using Two-Dimensional COLOC and HMBC Techniques

✍ Marie Kouda-Bonafos; Mouhoussine Nacro; Bernard Ancian 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 210 KB 👁 2 views

The 'H and "C NMR spectra of apigeninidin chloride were assigned utilizing the two-dimensional 6,, S, correlation and COLOC and HBMC techniques. The full 13C NMR data are reported for the first time. A good correlation was found between 13C chemical shifts and carbon charge obtained by AM1 calculati

Complete Signal Assignment of the 13C-NM

Complete Signal Assignment of the 13C-NMR Spectra and Structure Determination of Corrinoids by 2D-INADEQUATE and 2D 13C, 1H-NMR Chemical Shift Correlation Using nJ(C,H)

✍ Dr. Reinhard Benn; Dr. Richard Mynott 📂 Article 📅 1985 🏛 John Wiley and Sons 🌐 English ⚖ 345 KB 👁 2 views

Complete assignment of the 1H and 13C NM

Complete assignment of the 1H and 13C NMR spectra of 2-phenyl-3H-naphtho[2,1-b][1,4]oxazin-3-one, 2-p-methoxyphenylnaphtho[1,2-d]oxazole and 2-phenylnaphtho[1,2-d]oxazole. Concerted use of one- and two-dimensional NMR techniques

✍ Amelia Márquez; Claudio Saitz; Alvaro Cañete; Hernán Rodríguez; Carolina Jullian 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 223 KB 👁 1 views

The 1H and 13C NMR spectra of 2-phenyl-3H-naphtho [2,1-b][1,4]oxazin-3-one, 2-p-methoxyphenylnaphtho [1,2-d]oxazole and 2-phenylnaphtho[1,2-d]oxazole were totally assigned using a combination of one-and two-dimensional NMR techniques. In addition to correlation of the proton signals by a COSY spectr