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Structure determination of two conotoxins fromConus textile by a combination of matrix-assisted laser desorption/ionization time-of-flight and electrospray ionization mass spectrometry and biochemical methods

✍ Scribed by Kalume, D�rio E.; Stenflo, Johan; Czerwiec, Eva; Hambe, Bj�rn; Furie, Barbara C.; Furie, Bruce; Roepstorff, Peter

Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
222 KB
Volume
35
Category
Article
ISSN
1076-5174

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✦ Synopsis

Two highly modified conotoxins from the mollusc Conus textile, "-TxIX and Gla(1)-TxVI, were characterized by matrix-assisted laser desorption/ionization and electrospray mass spectrometry and also by electrospray ionization tandem and triple mass spectrometry in combination with enzymatic cleavage and chemical modification reactions. The mass spectrometric studies allowed the confirmation of the sequence determined by Edman degradation and assignment of unidentified amino acid residues, among which bromotryptophan residues and an O-glycosylated threonine residue were observed. Methyl esterification was found necessary for the site-specific assignment of the Gla residues in the peptides.

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