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Structure Determination of Membrane-Associated Proteins from Nuclear Magnetic Resonance Data

✍ Scribed by James D. Baleja

Publisher
Elsevier Science
Year
2001
Tongue
English
Weight
134 KB
Volume
288
Category
Article
ISSN
0003-2697

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✦ Synopsis

This Review covers the delineation and optimization of protein-lipid systems for study using solutionstate NMR spectroscopy. The first half presents the necessary background for a membrane protein biochemist to initiate collaboration with an NMR spectroscopist. The second half provides guidelines for the spectroscopist on data collection, analysis for obtaining conformational information, and structure generation and assessment. Although the emphasis is on the study of peptides in detergent micelles, methods are outlined for larger membrane-associated proteins and for use of other solubilizing agents.

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