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The three-dimensional solution structure of Aesculus hippocastanum antimicrobial protein 1 determined by 1H nuclear magnetic resonance

✍ Scribed by Franky Fant; Wim F. Vranken; Frans A.M. Borremans

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
538 KB
Volume
37
Category
Article
ISSN
0887-3585

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✦ Synopsis

Aesculus hippocastanum antimicrobial protein 1 (Ah-AMP1) is a plant defensin isolated from horse chestnuts. The plant defensins have been divided in several subfamilies according to their amino acid sequence homology. Ah-AMP1, belonging to subfamily A2, inhibits growth of a broad range of fungi. So far, a three-dimensional structure has been determined only for members of subfamilies A3 and B2. In order to understand activity and specificity of these plant defensins, the structure of a protein belonging to subfamily A2 is needed. We report the three-dimensional solution structure of Ah-AMP1 as determined from twodimensional 1 H nuclear magnetic resonance data. The structure features all the characteristics of the ''cysteine-stabilized ␣␤-motif.'' A comparison of the structure, the electrostatic potential surface and regions important for interaction with the fungal receptor, is made with Rs-AFP1 (plant defensin of subfamily A3). Thus, residues important for activity and specificity have been assigned.

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