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Structure and optical activity of the DNA–aminoacridine complex

✍ Scribed by Miss B. J. Gardner; S. F. Mason

Publisher
Wiley (John Wiley & Sons)
Year
1967
Tongue
English
Weight
912 KB
Volume
5
Category
Article
ISSN
0006-3525

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✦ Synopsis

The electronic absorption and circular dichroism spectra of the DNA-acridine orange complex have been measured over a range of ionic strength, pH, and DNA phosphate to dye (P/D) ratios. Three circular dichroism bands associated with the long wavelength absorption band of acridine orange are induced on complex formation with DNA. Two of the dichroism bands, due mainly to dimeric dye molecules, are favored by low ionic strength, low pH (3.2), and a low P/D ratio (-3), while the third, deriving primarily from monomeric dye, is optimnm a t high ionic strength, neutral pH, and a larger P / D ratio (9). The data suggest that monomeric acridine orange binds to DNA in the form of a left-handed helical array with four dye molecules per turn, while the bound dimer has a skewed sandwich conformation which is itself dissymmetric. The stereochemical relations between the bound monomer dye and the DNA are consistent with a modified intercalation model for tJhe DNA-acridine complex.

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