The binding curves of 1-, 2-, 3-, and 9-aminoacridine and proflavine on native DN.4 and the circular dichroism (CD) spectra of the bound cations have been determined under the same conditions. The variation of the CD spectra with the amount ( r ) of aminoacridine bound per DNA phosphorus waa of two
Induced optical activity of various aminoacridines bound to DNA
β Scribed by A. Blake; A. R. Peacocke
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1967
- Tongue
- English
- Weight
- 245 KB
- Volume
- 5
- Category
- Article
- ISSN
- 0006-3525
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The optical rotatory dispersion curves of the proflavine cation were measured in the spectral range 400-500 mp. No optical activity was observed for the free cation but a large positive Cotton effect appeared in the presence of DNA. The effect of ionic. strength, denaturation of the DNA, and the l)N
The electronic absorption and circular dichroism spectra of the DNA-acridine orange complex have been measured over a range of ionic strength, pH, and DNA phosphate to dye (P/D) ratios. Three circular dichroism bands associated with the long wavelength absorption band of acridine orange are induced
The absorption and rotatory properties of acridine orange-poly-Scarboxymethy1-Lcysteine system in water and in 0.2 M NaCl have been measured at different pH and polymer-to-dye mixing ratios. The absorption spectra indicate that the dyes are bound to the polymer in dimeric or highly aggregated forms.