Structure and expression of a cDNA encoding an RNA helicase-like protein in tobacco
โ Scribed by Hiraku Itadani; Mamoru Sugita; Masahiro Sugiura
- Publisher
- Springer
- Year
- 1994
- Tongue
- English
- Weight
- 415 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0167-4412
No coin nor oath required. For personal study only.
โฆ Synopsis
The human P68 protein is an ATP-dependent RNA helicase and thought to be involved in cell growth and division. We have isolated a Nicotiana syIvestris cDNA which encodes a p68-1ike protein. Northern blot analysis showed that the transcript from the gene is accumulated in N. sylvestris leaves, roots and flowers, but not in N. tabacum-cultured cells.
๐ SIMILAR VOLUMES
A cDNA encoding an RNA-binding protein (ribonucleoprotein or RNP) was isolated from a tobacco (Nicotiana sylvestris) cDNA library. The predicted protein (termed RGP-2) is 259 amino acids in length and consists of an N-terminal sequence of 39 amino acids, a consensus sequence type RNA-binding domain
Successful cloning with cDNA expression libraries involves interaction of ligand molecules (probes) with expressed fusion proteins of interest. So far those ligands leading to successful results fall into three classes: (i) antibodies, (ii) protein ligands that interact with the protein of interest,
Growth and differentiation of early embryos depends almost entirely on information which is maternally inherited in the form of macromolecules accumulated by the female gamete during its growth phase. Most of the maternal mRNAs synthesized by growing oocytes are not immediately recruited onto polyso
Photoinduction and photoinhibition of germination in seed from a homozygous tobacco (Nicotiana tabacum L.) line containing an introduced oat phyA eDNA (encoding phytochrome A) is compared with that of isogenic wild-type (WT) tobacco. Under continuous irradiation by a light source with a low red:far-