Photoregulation of germination in seed of transgenic lines of tobacco andArabidopsiswhich express an introduced cDNA encoding phytochrome A or phytochrome B
✍ Scribed by Alex C. McCormac; Harry Smith; Garry C. Whitelam
- Publisher
- Springer-Verlag
- Year
- 1993
- Tongue
- English
- Weight
- 918 KB
- Volume
- 191
- Category
- Article
- ISSN
- 0032-0935
No coin nor oath required. For personal study only.
✦ Synopsis
Photoinduction and photoinhibition of germination in seed from a homozygous tobacco (Nicotiana tabacum L.) line containing an introduced oat phyA eDNA (encoding phytochrome A) is compared with that of isogenic wild-type (WT) tobacco. Under continuous irradiation by a light source with a low red:far-red (R:FR) ratio the transgenic tobacco seed appeared to be less susceptible to photoinhibition of germination compared with WT seed. However, induction of germination following a short pulse by R (666 nm) was not enhanced in the genotype transformed by oat phyA eDNA compared with the WT; neither did germination of the transgenie tobacco seed show an increased sensitivity to saturating pulses of light of longer wavelengths (666-730 nm).
In seeds of transgenic Arabidopsis thaliana (L.) Heynh.
which contained an introduced phytochrome-B-encoding eDNA, levels of dark germination were enhanced, consistent with mediation of response by phytochrome B-Pfr. The germination behaviour of Arabidopsis genotypes wich contained an introduced eDNA encoding phytochrome A, however, did not significantly differ from that of the WT.