Structure and biosynthesis of Lyt-1

✍ Scribed by Jwu-Sheng Tung; Fung-Win Shen; Margaret C. Deere; Edward A. Boyse

• Book ID: 104741179
• Publisher: Springer-Verlag
• Year: 1984
• Tongue: English
• Weight: 496 KB
• Volume: 20
• Category: Article
• ISSN: 0093-7711
• DOI: 10.1007/bf00364484

✦ Synopsis

Two-dimensional chymotryptic peptide maps of Lyt-l.1 and Lyt-l.2 from Lyt-1 congenic thymocytes labeled with 125I in the usual way before lysis did not significantly differ, but there was a characteristic difference between maps of Lyt-l.1 and Lyt-l.2 obtained from a25I-labeled solubilized membrane fragments. We conclude that the Lyt-1 locus of chromosome 19 includes the protein-structural gene for Lyt-1. This conclusion is further supported by evidence with 35 S-cysteine-labeled thymocytes: Thus an early 62K intermediate form, and a 60K form from tunicamycin-treated cells devoid of N-linked and O-linked carbohydrates, were precipitated by Lyt-1 alloantibody, which implies that the allo-specificity of Lyt-1 glycoprotein (67K) resides partly or wholly in protein.