Structure and antitumor activity of an alkali-soluble polysaccharide from Cordyceps ophioglossoides

✍ Scribed by Haruki Yamada; Noboru Kawaguchi; Toshihiro Ohmori; Yasuyoshi Takeshita; Shin-ichi Taneya; Toshio Miyazaki

• Publisher: Elsevier Science
• Year: 1984
• Tongue: English
• Weight: 513 KB
• Volume: 125
• Category: Article
• ISSN: 0008-6215
• DOI: 10.1016/0008-6215(84)85146-0

✦ Synopsis

A water-insoluble extracellular glucan (CO-l) was isolated from the precipitate formed on incubation of the culture filtrate of Cordyceps ophioghsoides at 37' for 19 h. CO-1 was homogeneous as judged by h.p.l.c., electrophoresis, and ultracentrifugation, and the average molecular weight was determined by h.p.1.c. to be 632,000. The 'H-and i3C-n.m.r. and the i.r. spectra indicated that the glucosidic linkages in CO-1 were p. From the results of methylation analysis, Smith degradation, n.m.r. studies, and enzymic hydrolysis, it was concluded that CO-1 is composed of a backbone of (l-3)-linked p-D-glucopyranosyl residues with a P-Dglucopyranosyl group attached to O-6 of every second Dglucopyranosyl residue of the backbone. CO-1 strongly inhibited the growth of Sarcoma 180 solid-type tumor. CO-l polyalcohol, which was prepared by Smith degradation of CO-l, exhibited more-potent antitumor activity than CO-l. The absorption maximum of Congo Red shifted significantly in the presence of CO-l.