Structural studies of synthetic polynucleotides by polarographic techniques
β Scribed by Borek Janik; Ronald G. Sommer
- Book ID
- 103675317
- Publisher
- Elsevier Science
- Year
- 1976
- Tongue
- English
- Weight
- 765 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1567-5394
No coin nor oath required. For personal study only.
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The MCD (magnetic circular dichroism) spectra of Ap, ApA, ApApA, poly A, Up, UpU, poly U and double-stranded poly A:U alternating copoly A-U and alternating deoxyribopoly A-T were measured with a Cary 61 spectropolarimeter fitted with a Varian superconducting magnet at, a field strengt,h of 50 Kgaus
and Transfer RNA from E. co2i (strain B), and from yeast. At denaturation, peaks characteristic of the mononucleotide protons appear. Areas under the peaks can be used as a direct measure of denaturation. The sharpness of the spectral lines indicates that in the denatured form internal rotation abou