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Nuclear magnetic resonance study of synthetic polynucleotides and transfer RNA

✍ Scribed by John P. McTague; Virginia Ross; Julian H. Gibbs


Publisher
Wiley (John Wiley & Sons)
Year
1964
Tongue
English
Weight
469 KB
Volume
2
Category
Article
ISSN
0006-3525

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✦ Synopsis


and Transfer RNA from E. co2i (strain B), and from yeast. At denaturation, peaks characteristic of the mononucleotide protons appear. Areas under the peaks can be used as a direct measure of denaturation. The sharpness of the spectral lines indicates that in the denatured form internal rotation about the ribose-phosphate and the ribose-base links is relatively free. Linewidths also show that monomer units remain in either the ordered or freely rotating state for a t least lo-' see.


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Several 3-dehydro-(or ~-oxo-) ecdysteroids have been prepared by enzymatic and/or chemical means. Methods for their purification using various highperformance liquid chromatography systems are described. Proton and carbon nuclear magnetic resonance analyses show that 3-dehydroecdysteroids when disso