Nuclear magnetic resonance study of synthetic polynucleotides and transfer RNA
β Scribed by John P. McTague; Virginia Ross; Julian H. Gibbs
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1964
- Tongue
- English
- Weight
- 469 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0006-3525
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β¦ Synopsis
and Transfer RNA from E. co2i (strain B), and from yeast. At denaturation, peaks characteristic of the mononucleotide protons appear. Areas under the peaks can be used as a direct measure of denaturation. The sharpness of the spectral lines indicates that in the denatured form internal rotation about the ribose-phosphate and the ribose-base links is relatively free. Linewidths also show that monomer units remain in either the ordered or freely rotating state for a t least lo-' see.
π SIMILAR VOLUMES
Several 3-dehydro-(or ~-oxo-) ecdysteroids have been prepared by enzymatic and/or chemical means. Methods for their purification using various highperformance liquid chromatography systems are described. Proton and carbon nuclear magnetic resonance analyses show that 3-dehydroecdysteroids when disso