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Structural requirements of pyrroloquinoline quinone dependent enzymatic reactions

✍ Scribed by Arthur Oubrie; Bauke W. Dijkstra

Book ID
111753814
Publisher
Cold Spring Harbor Laboratory Press
Year
2000
Tongue
English
Weight
483 KB
Volume
9
Category
Article
ISSN
0961-8368

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πŸ“œ SIMILAR VOLUMES

Method of enzymatic determination of pyr
Method of enzymatic determination of pyrroloquinoline quinone
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An improved enzymatic method for the determination of pyrroloquinoline quinone, a novel prosthetic group of some important oxidoreductases, has been developed with cytoplasmic membrane of Escherichia coli K-12, in which ~-glucose dehydrogenase (EC 1.1.99.17) was completely resolved to apo-enzyme by

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Escherichia coli K-12 (ATCC 10 798) contains only the apoform of membrane-bound D-glucose dehydrogenase (pyrroloquinoline quinone-dependent). Crude membrane preparations or even crude cell extracts are suitable for a sensitive determination of pyrroloquinoline quinone under appropriate conditions. T