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Structural features of the cell-wall polysaccharides of Asparagus officinalis seeds

✍ Scribed by Renee Goldberg; Laurence Gillou; Roger Prat; Catherine Herve Du Penhoat; Veronique Michon

Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
915 KB
Volume
210
Category
Article
ISSN
0008-6215

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✦ Synopsis

The fine structure of a beta-)1----4)-linked glucomannan from Asparagus officinalis has been determined by n.m.r. analysis of the oligosaccharides obtained by acidic and enzymic hydrolyses. Cleavage of the glucomannan with beta-D-mannase from Aspergillus niger and purification by h.p.l.c. gave oligosaccharide fractions that contained Man (mannose), GlcMan (beta-glucopyranosylmannose), Man2, Glc2Man, and Glc3Man as the major components. Simulated digestion of a polymer composed of randomly distributed monomers with the same Glc:Man ratio as glucomannan from A. officinalis led to the same polysaccharides. The random distribution of the monomers of glucomannan from A. officinalis was corroborated by the diffraction diagram of the raw flour, which indicated that the "in situ" glucomannan was amorphous, whereas both cellulose and mannans are crystalline.

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