Two distinct forms of the 06 antigen (LPS) from E. coli were analysed using 'H and 13C NMR spectroseopoy. Their structures were found to be + 4)-P-D-Mang-( 1 + ~)-LY-D-G~c~NAc-( 1 + 4)-cy-p-GalpNAc-( 1 + 3)-p-~-Manp -( 1 + : X In the 06-specific polysaccharide from E. coli 06 : K2 and 06 : K13, X i
Structural comparison of the O4-specific polysaccharides from E. coli O4:K6 and E. coli O4:K52
โ Scribed by Barbara Jann; Alexander S. Shashkov; Helga Kochanowski; Klaus Jann
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 572 KB
- Volume
- 248
- Category
- Article
- ISSN
- 0008-6215
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โฆ Synopsis
Two distinct forms of the 04 antigen (LPS) from E. coli were analysed by 'H and '3C NMR spectroscopy. Both consisted of o-glucose, L-rhamnose, 2-acetamido-2,6-dideoxy_L-galactose (L-FucNAcl, and 2-acetamido-2-deoxy-o-glucose.
Their structures were found to be
In the 04-specific polysaccharide from E. coli 04:K3, G4:K6, and 04:K12, X is a-D-Glcp. In the 04 specific polysaccharide from E. coli 04: K52, the rhamnose residue is not substituted (X = Hl.
๐ SIMILAR VOLUMES
The capsular polysaccharide (K98 antigen) of E. coli O7:K98:H6 contains rhamnose, glucuronic acid, and acetate in the molar ratios 3:1:0.6. Methylation analysis, oligosaccharide analysis, and 1D- and 2D-n.m.r. spectroscopy revealed the polysaccharide to be a glucuronic acid-substituted rhamnan with
The polysaccharide moiety of the 022-antigen (lipopolysaccharide, LPS) consists of 2-acetamido-2deoxy-D-galactose, D-glucuronic acid, D-glucose, and D-galactose in the molar ratios 2: 1: 1: 1. Methylation analysis as well as 1D and 2D NMR spectroscopy showed that the 022 polysaccharide has the prima