Structural Characterization of Nuclear Poly(A)-Protein Particles in Rat Liver
✍ Scribed by Tihamér TOMCSÁNYI; János MOLNÁR; András TIGYI
- Book ID
- 115121669
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 587 KB
- Volume
- 131
- Category
- Article
- ISSN
- 1432-1327
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Poly(A) containing ribonucleoprotein particles were prepared from rat liver nuclei and polyribosomes. The particles have sedimentation coefficients of 14 S and 9 S, respectively. In CS2SO4 density gradients the particles banded at densities of 1.28-1.29 g cm-3. Both nuclear and polyribosomal poly(A)
Nuclear 14 S RNP particles containing poly (A) from Ehrlich ascites carcinoma cells and rat liver were purified by re-sedimentation in sucrose gradients, by Cs2SO4 density gradient centrifugation and by affinity chromatography on a poly (dT)-Sepharose column. Proteins of these RNP particles were ele