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Protein composition of nuclear 14Sribonucleoprotein particles containing poly (A)

✍ Scribed by J. Molnár; O. P. Samarina

Book ID
104738440
Publisher
Springer
Year
1975
Tongue
English
Weight
398 KB
Volume
2
Category
Article
ISSN
0301-4851

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✦ Synopsis

Nuclear 14 S RNP particles containing poly (A) from Ehrlich ascites carcinoma cells and rat liver were purified by re-sedimentation in sucrose gradients, by Cs2SO4 density gradient centrifugation and by affinity chromatography on a poly (dT)-Sepharose column. Proteins of these RNP particles were electrophoresed in urea and SDS-polyacrylamide gels. RNP particles of ascites carcinoma cells contain two main bands having molecular weights of 51 000 and 69 000 daltons, respectively, and two or three minor components.

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