𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Structural Changes of Casein Micelles in a Calcium Gradient Film

✍ Scribed by Ronald Gebhardt; Manfred Burghammer; Christian Riekel; Stephan Volkher Roth; Peter Müller-Buschbaum

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
236 KB
Volume
8
Category
Article
ISSN
1616-5187

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Calcium gradients are prepared by sequentially filling a micropipette with casein solutions of varying calcium concentration and spreading them on glass slides. The casein film is formed by a solution casting process, which results in a macroscopically rough surface. Microbeam grazing incidence small‐angle X‐ray scattering (µGISAXS) is used to investigate the lateral size distribution of three main components in casein films: casein micelles, casein mini‐micelles, and micellar calcium phosphate. At length scales within the beam size the film surface is flat and detection of size distribution in a macroscopic casein gradient becomes accessible. The model used to analyze the data is based on a set of three log‐normal distributed particle sizes. Increasing calcium concentration causes a decrease in casein micelle diameter while the size of casein mini‐micelles increases and micellar calcium phosphate particles remain unchanged.

magnified image

📜 SIMILAR VOLUMES

The conformation and aggregation of bovi
The conformation and aggregation of bovine β-casein A. II. Thermodynamics of thermal association and the effects of changes in polar and apolar interactions on micellization
✍ M. T. A. Evans; M. C. Phillips; M. N. Jones 📂 Article 📅 1979 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 972 KB

## Abstract A sedimentation analysis has been used to determine the proportion of protein present as monomer and aggregate in 0.5 and 1.0 g/dl solutions of β‐casein A in pH 7 phosphate buffer over the temperature range 10–40°C. The amount and molecular weight of the aggregate increase with temperat