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Structural and Thermodynamic Characterization of T4 Lysozyme Mutants and the Contribution of Internal Cavities to Pressure Denaturation

✍ Scribed by Ando, Nozomi; Barstow, Buz; Baase, Walter A.; Fields, Andrew; Matthews, Brian A.; Gruner, Sol M.


Book ID
122891469
Publisher
Biophysical Society
Year
2009
Tongue
English
Weight
52 KB
Volume
96
Category
Article
ISSN
0006-3495

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Contributions of all 20 amino acids at s
✍ Blaine H. M. Mooers; Walter A. Baase; Jonathan W. Wray; Brian W. Matthews πŸ“‚ Article πŸ“… 2009 πŸ› Cold Spring Harbor Laboratory Press 🌐 English βš– 867 KB

## Abstract To try to resolve the loss of stability in the temperature‐sensitive mutant of T4 lysozyme, Arg 96 β†’ His, all of the remaining 18 naturally occurring amino acids were substituted at site 96. Also, in response to suggestions that the charged residues Lys85 and Asp89, which are 5–8 Γ… away