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Structural and thermodynamic analysis of the binding of solvent at internal sites in T4 lysozyme

✍ Scribed by Jian Xu; Walter A. Baase; Michael L. Quillin; Enoch P. Baldwin; Brian W. Matthews


Book ID
119982921
Publisher
Cold Spring Harbor Laboratory Press
Year
2001
Tongue
English
Weight
720 KB
Volume
10
Category
Article
ISSN
0961-8368

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## Abstract To try to resolve the loss of stability in the temperature‐sensitive mutant of T4 lysozyme, Arg 96 β†’ His, all of the remaining 18 naturally occurring amino acids were substituted at site 96. Also, in response to suggestions that the charged residues Lys85 and Asp89, which are 5–8 Γ… away