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Structural and functional consequences of removal of the interdomain disulfide bridge from the isolated C-lobe of ovotransferrin

✍ Scribed by B. K. Muralidhara; Masaaki Hirose


Book ID
111753837
Publisher
Cold Spring Harbor Laboratory Press
Year
2000
Tongue
English
Weight
388 KB
Volume
9
Category
Article
ISSN
0961-8368

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## Abstract The engineering of lipase B from __Candida antarctica__ (CALB) by circular permutation has yielded over sixty hydrolase variants, and several show significantly improved catalytic performance. Here we report a detailed characterization of ten selected enzyme variants by kinetic and spec