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Removal of the conserved disulfide bridges from the scfv fragment of an antibody: effects on folding kinetics and aggregation

✍ Scribed by Kathrin Ramm; Peter Gehrig; Andreas Plückthun


Book ID
115629399
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
237 KB
Volume
290
Category
Article
ISSN
0022-2836

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The binding of the Fab fragment of monoclonal antibody NC10 to influenza virus N9 neuraminidase, isolated from tern and whale, was measured using an optical biosensor. Both neuraminidases, homotetramers of 190 kDa, were immobilized to avoid multivalent binding, and the binding of the monovalent NC10