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Structural and biochemical characterization of a nitrilase from the thermophilic bacterium,Geobacillus pallidusRAPc8

✍ Scribed by Dael S. Williamson; Kyle C. Dent; Brandon W. Weber; Arvind Varsani; Joni Frederick; Robert N. Thuku; Rory A. Cameron; Johan H. van Heerden; Donald A. Cowan; B. Trevor Sewell


Publisher
Springer
Year
2010
Tongue
English
Weight
958 KB
Volume
88
Category
Article
ISSN
1432-0614

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The Azorobucrer-type 7Fe ferredoxin from Bacillus schlegelii was active as an electron carrier in the reduction system of cyt. c consisting of NADPH, FNR and cytochrome c (cyt. c). The only [ 3Fe\_4S] cluster in the Fd molecule acts as the active site for the reduction of cyt. c. The activity of the