Structural Analysis of the Capsular Polysaccharide from Acinetobacter lwoffii F78

✍ Scribed by Anna Hanuszkiewicz; Zbigniew Kaczyński; Buko Lindner; Torsten Goldmann; Ekkehard Vollmer; Jennifer Debarry; Holger Heine; Otto Holst

• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 224 KB
• Volume: 2008
• Category: Article
• ISSN: 1434-193X
• DOI: 10.1002/ejoc.200800887

✦ Synopsis

Abstract

The capsular polysaccharide from Acinetobacter lwoffii F78 was isolated and purified, and its structure was elucidated by chemical analyses, NMR spectroscopy, and mass spectrometry. The presence of a capsule on this bacterium was confirmed by transmission electron microscopy experiments, and the utilization of the antilipid A monoclonal antibody proved the nonendotoxin origin of the isolated material. The structure represents a novel nonbranched aminopolysaccharide with high heterogeneity of amino groups substituents: →3)‐α‐L‐FucNAc‐(1→3)‐β‐D‐QuiNR^1^4NR^2^‐(1→4)‐β‐L‐GlcNR^3^3NR^4^A‐(1→, where R^1^ = 3‐hydroxybutyric acid (3‐HBA) or N‐acetylated alanine (AlaNAc), R^2^ = AlaNAc or 3‐HBA, R^3^ = acetyl or 3‐HBA, and R^4^ = 3‐HBA or acetyl.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2008)