Structural and Immunochemical Analysis of the Lipopolysaccharide from Acinetobacter lwoffii F78 Located Outside Chlamydiaceae with a Chlamydia-Specific Lipopolysaccharide Epitope
✍ Scribed by Anna Hanuszkiewicz; Göran Hübner; Evgeny Vinogradov; Buko Lindner; Lore Brade; Helmut Brade; Jennifer Debarry; Holger Heine; Otto Holst
- Publisher
- John Wiley and Sons
- Year
- 2008
- Tongue
- English
- Weight
- 448 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0947-6539
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✦ Synopsis
Abstract
Chemical analyses, NMR spectroscopy, and mass spectrometry were used to elucidate the structure of the rough lipopolysaccharide (LPS) isolated from Acinetobacter lwoffii F78. As a prominent feature, the core region of this LPS contained the disaccharide α‐Kdo‐(2→8)‐α‐Kdo (Kdo=3‐deoxy‐d‐D‐manno‐oct‐2‐ulopyranosonic acid), which so far has been identified only in chlamydial LPS. In serological investigations, the anti‐chlamydial LPS monoclonal antibody S25–2, which is specific for the epitope α‐Kdo‐(2→8)‐α‐Kdo, reacted with A. lwoffii F78 LPS. Thus, an LPS was identified outside Chlamydiaceae that contains a Chlamydia‐specific LPS epitope in its core region.