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Structural analysis of mutant hen egg-white lysozyme preferring a minor binding mode

โœ Scribed by Katsumi Maenaka; Masaaki Matsushima; Gota Kawai; Kimitsuna Watanabe; Ryota Kuroki; Izumi Kumagai


Book ID
114149583
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
508 KB
Volume
1384
Category
Article
ISSN
0167-4838

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Binding mode of N,Nโ€ฒ,Nโ€ณ, Nโ€ฒโ€ณ-tetraacetyl
โœ Tamo Fukamizo; Takeshi Ohkawa; Yasuo Ikeda; Takao Torikata; Sachio Goto ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 395 KB

The binding of N, N',N",N" to hen egg white lysozyme was investigated by fluorescence and 1H NMR spectroscopy. From observation of changes in the fluorescence intensity, the association constants of (GlcNAc)4-ol and (GlcNAc) 3 were found to be 0.70 x 105 and 1.07 x 105 M -1, respectively, at pH 5.0