Strong influence of hierarchically structured diamond nanotopography on adhesion of human osteoblasts and mesenchymal cells

✍ Scribed by Broz, A. ;Baresova, V. ;Kromka, A. ;Rezek, B. ;Kalbacova, M.

• Book ID: 105365650
• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 447 KB
• Volume: 206
• Category: Article
• ISSN: 0031-8965
• DOI: 10.1002/pssa.200982203

✦ Synopsis

Abstract

We investigate the influence of different roughnesses (20, 270, and 500 nm) of silicone substrates hermetically coated with the same nanocrystalline diamond (NCD) films on adhesion (1 h) and viability (48 h) of human mesenchymal stromal cells (MSC) and human osteoblasts (SAOS‐2). MSC adhere similarly on all NCD surfaces and control polystyrene (PS), however, their metabolic activity on NCD surfaces is increased. Osteoblasts adhere on NCD in significantly higher numbers than on PS and their adhesion is inversely proportional to increasing substrate roughness. Their metabolic activity is decreased on nano/microrough NCD surfaces in contrast to MSC. The data show that NCD is a suitable biomaterial and can control cell adhesion and growth.