𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Stretch-mediated responses of osteoblast-like cells cultured on titanium-coated substratesin vitro

✍ Scribed by Walboomers, X. F. ;Habraken, W. J. E. M. ;Feddes, B. ;Winter, L. C. ;Bumgardner, J. D. ;Jansen, J. A.

Publisher
John Wiley and Sons
Year
2004
Tongue
English
Weight
494 KB
Volume
69A
Category
Article
ISSN
0021-9304

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Cyclic stretching experiments on osteoblast‐like cells have proven to be a useful tool in understanding the underlying mechanisms of load transduction at the bone–implant surface. However, most experimental setups use silicone rubber substrates, which are atypical for orthopedic and dental implant materials. Therefore, we investigated the responses of osteoblast‐like cells to loading on titanium (Ti)‐coated versus plain silicone substrates. Ti‐coated substrates were made by a radio‐frequency magnetron sputtering process, and characterized using Rutherford backscattering spectrometry, X‐ray photoelectron spectroscopy, and contact‐angle measurements. Osteoblast‐like cells cultured from rat bone marrow were seeded on both types of substrates and stretched for 1 h continuously. Subsequently, cell proliferation, alkaline phosphatase activity, and calcium content were measured for up to 24 days after seeding. In addition light‐, scanning electron‐, and confocal laser scanning micrographs were made. The results showed that our Ti coating had a thickness of 50 nm and contained Ti/oxygen as 1:1. However, further characterization proved that the silicone material had a tendency to resurface through the coating. Osteoblast‐like cells proliferated faster on the Ti‐coated substrates, but differentiation was slower compared with the silicone substrates. It was concluded that that there was a definitive influence of the substrate material in mechanical stress models. Therefore, extrapolation of results obtained using silicone substrates cannot be translated directly toward the situation of metallic implant materials. © 2004 Wiley Periodicals, Inc. J Biomed Mater Res 69A: 131–139, 2004

📜 SIMILAR VOLUMES

The response of osteoblast-like cells to
The response of osteoblast-like cells towards collagen type I coating immobilized by p-nitrophenylchloroformate to titanium
✍ Juliette van den Dolder; John A. Jansen 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 290 KB

## Abstract The scaffold surface composition can be altered by the use of surface coatings. The use of thin coatings will give special surface properties, while the bulk properties of the scaffold are preserved. Collagen type I is known to play an important role during cell adhesion as well as oste

In vitro kinetic study of growth and min
In vitro kinetic study of growth and mineralization of osteoblast-like cells (Saos-2) on titanium surface coated with a RGD functionalized bisphosphonate
✍ Johanne Beuvelot; David Portet; Grégory Lecollinet; Marie-Françoise Moreau; Mich 📂 Article 📅 2009 🏛 John Wiley and Sons 🌐 English ⚖ 314 KB 👁 1 views

## Abstract Osteoconduction and osseointegration are the critical stages for implantation success. Peptides containing RGD (Arg‐Gly‐Asp) adhesive sequence are known to promote cell adhesion and consequently to favor osseointegration of medical devices. In this study, RGD peptides were coupled to a

Prostaglandins mediate the effects of ti
Prostaglandins mediate the effects of titanium surface roughness on MG63 osteoblast-like cells and alter cell responsiveness to 1?,25-(OH)2D3
✍ Batzer, R. ;Liu, Y. ;Cochran, D. L. ;Szmuckler-Moncler, S. ;Dean, D. D. ;Boyan, 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 240 KB 👁 2 views

Surface roughness affects proliferation, differentiation (alkaline phosphatase and osteocalcin), local factor production [transforming growth factor (TGF␤) and prostaglandin E 2 (PGE 2 )], and response to 1,25-(OH) 2 D 3 (1,25) of MG63 osteoblast-like cells. In this study, we examined whether the ef

Variation of oxide films on titanium ind
Variation of oxide films on titanium induced by osteoblast-like cell culture and the influence of an H2O2 pretreatment
✍ Pan, J. ;Liao, H. ;Leygraf, C. ;Thierry, D. ;Li, J. 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 964 KB

Variations of titanium oxide films induced by osteoblast-like cells in a rat calvaria culture system and the influence of an H 2 O 2 pretreatment have been investigated by using X-ray photoelectron spectroscopy and electrochemical impedance spectroscopy. For abraded titanium, the results revealed th