In vitro kinetic study of growth and mineralization of osteoblast-like cells (Saos-2) on titanium surface coated with a RGD functionalized bisphosphonate
✍ Scribed by Johanne Beuvelot; David Portet; Grégory Lecollinet; Marie-Françoise Moreau; Michel Félix Baslé; Daniel Chappard; Hélène Libouban
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 314 KB
- Volume
- 90B
- Category
- Article
- ISSN
- 1552-4973
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✦ Synopsis
Abstract
Osteoconduction and osseointegration are the critical stages for implantation success. Peptides containing RGD (Arg‐Gly‐Asp) adhesive sequence are known to promote cell adhesion and consequently to favor osseointegration of medical devices. In this study, RGD peptides were coupled to a bisphosphonate used as an anchor system and chemically adsorbed on polished titanium discs. Two different concentrations, 10^−10^ mol/L (RGD 10^−10^) and 10^−4^ mol/L (RGD 10^−4^) were compared to non coated discs (RGD 0). Adhesion, spreading, and mineralization of osteoblast‐like cells (Saos‐2) were assessed. Mineralization kinetic was done at 3, 6, 10, 14, and 18 days of culture; the extent of mineral deposits was quantified by image analysis. Histogram repartitions of nuclear area, characterizing cell spreading, showed a shift to higher values in cells cultured on RGD coated titanium disks. Mineralization started at day 3 in the three groups, but had a faster development in the RGD 10^−10^ group from day 6 to day 18 compared to RGD 0 and RGD 10^−4^. At day 18, the percentage of mineralized area was significantly higher for RGD 10^−10^ compared to RGD 0 (p < 0.05). In the present study, this new method was found suitable to anchor RGD containing species on titanium: this favored adhesion and spreading of osteoblast‐like cells and mineralization compared to noncoated titanium. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 2009