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Strategies to maximize heterologous protein expression in Escherichia coli with minimal cost

✍ Scribed by Wolfgang Peti; Rebecca Page

Book ID
116828439
Publisher
Elsevier Science
Year
2007
Tongue
English
Weight
311 KB
Volume
51
Category
Article
ISSN
1046-5928

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Nuclear magnetic resonance is an important tool for high-resolution structural studies of proteins. It demands high protein concentration and high purity; however, the expression of proteins at high levels often leads to protein aggregation and the protein purification step can correspond to a high