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A screening strategy for heterologous protein expression in Escherichia coli with the highest return of investment

✍ Scribed by Benny Pacheco; Lissete Crombet; Peter Loppnau; Doug Cossar

Book ID
116829396
Publisher
Elsevier Science
Year
2012
Tongue
English
Weight
752 KB
Volume
81
Category
Article
ISSN
1046-5928

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Nuclear magnetic resonance is an important tool for high-resolution structural studies of proteins. It demands high protein concentration and high purity; however, the expression of proteins at high levels often leads to protein aggregation and the protein purification step can correspond to a high