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Strand Specificity of DNA Unwinding by RecBCD Enzyme

✍ Scribed by Gary Braedt and Gerald R. Smith


Book ID
123636603
Publisher
National Academy of Sciences
Year
1989
Tongue
English
Weight
893 KB
Volume
86
Category
Article
ISSN
0027-8424

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Interaction of RecBCD enzyme with DNA da
✍ BrčiΔ‡-KostiΔ‡, Krunoslav ;Salaj-Ε mic, Erika ;MarΕ‘iΔ‡, NataΕ‘a ;KajiΔ‡, Sanja ;Stojil πŸ“‚ Article πŸ“… 1991 πŸ› Springer 🌐 English βš– 725 KB

The DNA of a gene 2 mutant (T4 2-) of phage T4 is degraded by RecBCD enzyme in the bacterial cytoplasm. Under normal conditions, recBCD+ cells are therefore incapable of supporting the growth of phage T4 2-. Only if the nucleolytic activity of RecBCD enzyme is absent from the cytoplasm are T4 2(-)-i