Inhibition of RecBCD Enzyme by Antineoplastic DNA Alkylating Agents
β Scribed by Barbara Dziegielewska; Terry A. Beerman; Piero R. Bianco
- Book ID
- 116663154
- Publisher
- Elsevier Science
- Year
- 2006
- Tongue
- English
- Weight
- 821 KB
- Volume
- 361
- Category
- Article
- ISSN
- 0022-2836
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The DNA of a gene 2 mutant (T4 2-) of phage T4 is degraded by RecBCD enzyme in the bacterial cytoplasm. Under normal conditions, recBCD+ cells are therefore incapable of supporting the growth of phage T4 2-. Only if the nucleolytic activity of RecBCD enzyme is absent from the cytoplasm are T4 2(-)-i
## Abstract DNA damage was evaluated by flow cytometric (FCM) analysis of cells treated with Lβphenylalanine mustard (LβPAM) and stained with antiβDNA monoclonal antibody (MAb) F7β26. DNA damage was rapidly repaired, as indicated by the loss of DNA immunoreactivity after removal of LβPAM. Two types