Stored of Hsp72/Hsp73 in Germinal Vesicle-stage Mouse Oocytes

✍ Scribed by C-H Liu; C-C Yang; DP-C Lin; M-H Wu; K-J Tsai

• Book ID: 104468637
• Publisher: John Wiley and Sons
• Year: 2004
• Tongue: English
• Weight: 124 KB
• Volume: 39
• Category: Article
• ISSN: 0936-6768
• DOI: 10.1046/j.1439-0531.2003.00470.x

✦ Synopsis

Contents

Heat‐shock proteins (hsps) Hsp72 and Hsp73 are the stored maternal proteins found in mouse oocytes. Both hsps appear in mouse oocytes at germinal vesicle (GV) and metaphase II (M‐II)‐stages as previously demonstrated by immunoblotting analysis. In this report, we further determined the presences of Hsp72/Hsp73 proteins in mouse embryos at stages of 2‐pronucleus, arrested 1‐cell, 2‐cell, arrested 2‐cell, 4‐cell, arrested 4‐cell, 8‐cell to morula and blastocyst. Except for the blastocyst stage, the Hsp72/Hsp73 proteins were detectable in most embryo stages. The concentration of Hsp72/Hsp73 in GV‐stage oocytes was higher than that in M‐II‐stage oocytes, and in any stages of embryos before implantation. A dramatical increase in Hsp72/Hsp73 expression was found at the 2‐cell stage. Together with these findings, we speculated that hsps accumulated or stored earlier in the GV‐stage mouse oocytes to protect the oocytes against environmental influences acting on ovary, and hsps may be required for zygotic gene activation and provided a protective effect against apoptosis.