Inhibiting Phosphodiesterase Activity to Maintain Germinal Vesicle Stage Arrest Inhibits DNA Strand Breaks in Germinal Vesicle Stage Mouse Oocytes

High developmental competence of cattle

High developmental competence of cattle oocytes maintained at the germinal vesicle stage for 24 hours in culture by specific inhibition of MPF kinase activity

✍ Pascal Mermillod; Milan Tomanek; Réjane Marchal; Laurent Meijer 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 152 KB

Roscovitine, a potent inhibitor of M-phase Promoting Factor (MPF) kinase activity, was used to maintain cattle oocytes at the germinal vesicle stage for a 24-hr culture period. A concentration of 25 M of roscovitine was sufficient to reach the maximum level of meiotic resumption inhibition with 83 Ϯ