Stopped-flow chemiluminescence spectrometry to improve the determination of pencillins based on the luminol-iodine reaction

✍ Scribed by Sebastián Ventura; Manuel Silva; Dolores Pérez-Bendito

• Publisher: Elsevier Science
• Year: 1992
• Tongue: English
• Weight: 626 KB
• Volume: 266
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(92)85056-c

✦ Synopsis

The hght generated m the reaction between lummol and &me was used for the m&rect stopped-flow chemdummescence spectrometnc determmatlon of pemcdlins based m their mhlbltory effect on this reactlon The reation was momtored v1a direct rate measurements on the formatron and decay steps of the chenulummescence process m addrtron to peak-height measurements that were made for compmn The proposed method allows the determmafion of pemcdhns m the range 1 X 10m6-1 2 X 10m4 M Hnth a relatwe standard donation of ca 2% and a samphng frequency of 120 h-' The method IS relatnrely free from the mterference of common exclplents accompanymg pemcdhns m pharmaceutical samples Its figures of merrt (preclslon, rapldlty, sample consumption and hablllty to automation) compare favourably with those of its batch counterpart, which entads a chenuluounescence titration assay xeywords Chenuhmunescence, Fluonmetry, Pemcdlms

Chemdummescence (CL) results from hght bemg enutted m a chenucal reaction Until recently, chemdummescent reactions were regarded merely as mterestmg phenomena and few attempts at developmg analytxcal chenxal apphcatlons based on them had been made However, the development of extremely sensltlve and rehable mstrumentatlon has aroused much interest m such reactlons lately [1,2] The wide vanety of such apphcatlons currently avadable testify to the remarkable sensltlvlty and selectlvlty of CL reactions 111 several areas of mterest In this context, apphcatlons to drug analysis are stdl few m number but are growmg steaddy [3-51 Recent work m this laboratory mvolved the search for CL