Stopped-flow analysis of Ru(bpy)33+chemiluminescent reactions

✍ Scribed by Shultz, Loranelle L. ;Nieman, Timothy A.

• Book ID: 101289800
• Publisher: John Wiley and Sons
• Year: 1998
• Weight: 131 KB
• Volume: 13
• Category: Article
• ISSN: 0884-3996
• DOI: 10.1002/(sici)1099-1271(199803/04)13:2<85::aid-bio470>3.0.co;2-w

✦ Synopsis

The stopped-¯ow technique was employed to measure chemiluminescent emission from the reaction of a mixture of oxalate and proline with a chemiluminescence reagent, tris(2,2'bipyridine)ruthenium(III), or Ru(bpy) 3 3 . Ru(bpy) 3 3 is a versatile reagent and is often used in bioanalytical applications, including the detection of certain drugs and their metabolites, for example. Unfortunately, Ru(bpy) 3 3 has not yet been fully examined as a possible chemiluminescence reagent for simultaneous kinetic determinations. In this work, a differential reaction rate method, based on simple least squares regressions of the pseudo-®rst order decay data, was used to resolve two compounds, oxalate and proline, reacting simultaneously with Ru(bpy) 3 3 . Our results indicate that stopped-¯ow analyses with Ru(bpy) 3 3 could provide a viable method for simultaneous determinations of unresolvable analytes of environmental and pharmaceutical importance.