Stoichiometry, Kinetics, and Regulation of Glucose and Amino Acid Metabolism of a Recombinant BHK Cell Line in Batch and Continuous Cultures
โ Scribed by Monika Linz; An-Ping Zeng; Roland Wagner; Wolf-Dieter Deckwer
- Book ID
- 109387969
- Publisher
- American Institute of Chemical Engineers
- Year
- 1997
- Tongue
- English
- Weight
- 287 KB
- Volume
- 13
- Category
- Article
- ISSN
- 8756-7938
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
## Abstract The suitability of an established myogenic line (L~6~) for the study of skeletal muscle intermediary metabolism was investigated. Myoblasts were grown in tissue culture for ten days at which time they had differentiated into multinucleated myotubes. Myotube preparations were then incuba
The analysis of free amino acids in mammalian cell culture media can give valuable information on the metabolism of particular commercially valuable cell lines. Analysis of spent culture media indicates potential limiting nutrients. However, analysis over the whole culture period enables a kinetic a