Stimulation of somatic embryogenesis in carrot cells by the addition of calcium
β Scribed by Toshiya Takeda; Hideyuki Inose; Hiroshi Matsuoka
- Publisher
- Elsevier Science
- Year
- 2003
- Tongue
- English
- Weight
- 167 KB
- Volume
- 14
- Category
- Article
- ISSN
- 1369-703X
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We report the identification, via their cDNAs, of genes which are temporarily transcribed during the initiation of somatic embryogenesis in carrot (Daucus carota L.) cells cultured in an auxin-free medium. Their expression is roughly associated with the first morphogenetic, or globular, stage. A cDN
The pattern of expression of the rolB gene, derived from the T-DNA of the plant pathogen Agrobacterium rhizogenes, has been investigated during the early stages of somatic embryo formation in suspension cultures of carrot (Daucus carota L.). The reporter gene GUS (Escherichia coli -glucuronidase), u
Roots of nine cultivars of carrot (Daucus carom L.) were exposed to one, two or three months cold treatment and cells isolated from cold-treated and control roots were assayed for the production of somatic embryos. Cells obtained from the one-or two-months cold treatments formed embryos earlier, pro
An upward shift in the concentration of calcium present in the medium during somatic embryogenesis increased the number of embryos produced approximately two-fold. This was observed when embryogenic suspension cells grown in 2,4-D medium with the normal calcium concentration of 10(-3) M were transfe