Gene expression during induction of somatic embryogenesis in carrot cell suspensions

✍ Scribed by F. Aleith; G. Richter

• Publisher: Springer-Verlag
• Year: 1991
• Tongue: English
• Weight: 846 KB
• Volume: 183
• Category: Article
• ISSN: 0032-0935
• DOI: 10.1007/bf00197562

✦ Synopsis

We report the identification, via their cDNAs, of genes which are temporarily transcribed during the initiation of somatic embryogenesis in carrot (Daucus carota L.) cells cultured in an auxin-free medium. Their expression is roughly associated with the first morphogenetic, or globular, stage. A cDNA library (2 gt 10) was established using poly(A)+-rich RNAs from cells deprived of auxin for 8 d. By differential screening a number of clones corresponding to early-induced embryogenic genes were identified. For several a temporary accumulation of the specific mRNA between 6 and 16 d after induction was observed. With regard to the nucleotide sequence and the respective deduced amino-acid sequence, two glycine-rich proteins and a polypeptide with a proline-rich domain were among the products of genes activated at the onset of somatic embryogenesis.