The effect of retinoic acid (RA) on the expression of myelin-specific genes, i.e., proteolipid protein (PLP) and myelin-associated glycoprotein (MAG) in rat glioma C6 cells, was analyzed by Northern blot hybridization. RA-treatment increased the steady-state level of the PLP-specific messages within
Stimulation of myelin proteolipid protein gene expression by eicosapentaenoic acid in C6 glioma cells
β Scribed by Serafina Salvati; Francesco Natali; Lucilla Attorri; Carla Raggi; Antonella Di Biase; Massimo Sanchez
- Book ID
- 117410011
- Publisher
- Elsevier Science
- Year
- 2004
- Tongue
- English
- Weight
- 190 KB
- Volume
- 44
- Category
- Article
- ISSN
- 0197-0186
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## Abstract A Model culture system of C6 rat glioma cells was used to test the involvement of cAMP in the regulation of the myelin PLP and MAG genes. The treatment of cells with isoproterenol (10^β5^to 10^β8^M) upregulated the expression of the PLP and MAG genes in a concentrationβdependent manner.
The effect of dexamethasone on the expression of proteolipid protein (PLP) and myelin-associated glycoprotein (MAG) genes was investigated in rat C6 glioma cells. The steady state level of the respective mRNAs was quantitated by Northern blot analysis. The treatment of cells with dexamethasone trans