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Glucocorticoid-induced upregulation of proteolipid protein and myelin-associated glycoprotein genes in C6 cells

✍ Scribed by W. Zhu; R. C. Wiggins; Dr G. W. Konat

Publisher: John Wiley and Sons
Year: 1994
Tongue: English
Weight: 521 KB
Volume: 37
Category: Article
ISSN: 0360-4012
DOI: 10.1002/jnr.490370206

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✦ Synopsis

The effect of dexamethasone on the expression of proteolipid protein (PLP) and myelin-associated glycoprotein (MAG) genes was investigated in rat C6 glioma cells. The steady state level of the respective mRNAs was quantitated by Northern blot analysis. The treatment of cells with dexamethasone transiently upregulated the expression of both genes with peak mRNA levels of approximately 10-fold over control levels occurring at day 3 for the PLP gene and at day 5 for the MAG gene. The efTect was directly related to the drug concentration in the range from lop9 to M. Combined exposure of the cells to dexamethasone and retinoic acid featured an additive effect on PLP gene expression, whereas MAG gene expression was depressed below detectability level. The dissimilarity in the response of the genes to dexamethasone and retinoic acid supports the contention that the genes are controlled by different mechanisms. Furthermore, the results indicate that the effects of dexamethasone and retinoic acid on the myelin genes are mediated by different regulatory pathways.

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