Stimulation of human monocyte chemiluminescence by staphylococcal lipoteichoic acid
โ Scribed by Y. Ohshima; J. Beuth; A. Yassin; H. L. Ko; G. Pulverer
- Publisher
- Springer-Verlag
- Year
- 1988
- Tongue
- English
- Weight
- 326 KB
- Volume
- 177
- Category
- Article
- ISSN
- 0300-8584
No coin nor oath required. For personal study only.
โฆ Synopsis
Lipoteichoic acid (LTA) from Staphylococcus saprophyticus strains S1 and S35 was shown to be a potent stimulator of human monocyte chemiluminescence, whereas human granulocytes were not activated. Data on the chemical analysis of these LTA-preparations were given.
๐ SIMILAR VOLUMES
Maximal rates of 0 ; and H,02 production by human bloodstream monocytes activated during the respiratory burst by phorbol ester were only about 10% of those of neutrophils. Furthermore, monocytes possess only about 5% of the myeloperoxidase activity of neutrophils and so can only produce l o w level
DD1 9SY. UK Human mononuclear cells were separated from freshly obtained peripheral venous blood by density centrifugation and the number of monocytes present estimated by volume spectroscopy. The mononuclear cells were then placed directly into the wells of a microtitre plate and incubated for one