Chemiluminescence of human bloodstream monocytes and neutrophils: An unusual oxidant(s) generated by monocytes during the respiratory burst
✍ Scribed by Davies, Bernard ;Edwards, Steven W.
- Publisher
- John Wiley and Sons
- Year
- 1992
- Weight
- 826 KB
- Volume
- 7
- Category
- Article
- ISSN
- 0884-3996
No coin nor oath required. For personal study only.
✦ Synopsis
Maximal rates of 0 ; and H,02 production by human bloodstream monocytes activated during the respiratory burst by phorbol ester were only about 10% of those of neutrophils. Furthermore, monocytes possess only about 5% of the myeloperoxidase activity of neutrophils and so can only produce l o w levels of HOCl and related compounds. These combined reductions in 0; generating ability and lower myeloperoxidase levels result in low levels of luminol chemiluminescence stimulated during the respiratory burst of monocytes. However, although monocytes generate much lower levels of 0 ; and H202 than neutrophils, these cells produce comparable rates of PMA-stimulated lucigenin chemiluminescence. Hence, this assay does not accurately reflect the production of either of these t w o oxidants by activated phagocytes, and further lucigenin must react with some other oxidant(s) via a process which leads t o photon emission. This oxidant(s) is not O;, H,O,, * OH, '02 or NO, but is derived from 0; generated during the respiratory burst and is generated in greater quantities by activated monocytes compared w i t h neutrophils. Thus, lucigenin chemiluminescence is an indirect measure o f superoxide release.