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Stimulation of energy metabolism by α-adrenergic agonists in primary cultures of astrocytes

✍ Scribed by K. V. Subbarao; Dr. L. Hertz

Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
672 KB
Volume
28
Category
Article
ISSN
0360-4012

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✦ Synopsis

Noradrenaline effects on glucose oxidation were studied in primary cultures of astrocytes. C 0 2 formation from labeled glucose was enhanced in the presence of noradrenaline. The stimulatory effect by noradrenaline was exerted both on lactate formation (-20%) and on tricarboxylic acid activity (CO, production from glutamate) (-40%). The effect was, at least partly, exerted on the alpha-ketoglutarate dehydrogenase step. The EC,, value for noradrenaline on lactate formation was significantly lower (60 nM) than that on oxidative metabolism (1,900 nM). Studies with specific adrenergic agonists and antagonists showed that various receptor subtypes are involved. Thus, the effect on lactate formation was mediated exclusively by stimulation of an alpha, receptor whereas oxidative metabolism was enhanced by both alpha, and alpha, receptor stimulation. No effects were exerted by beta receptor agonists or antagonists.

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