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Characterization of Gsα mRNA transcripts in primary cultures of rat brain astrocytes

✍ Scribed by Douglas L. Feinstein; Susanne M. Mumby; Robert J. Milner

Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
707 KB
Volume
5
Category
Article
ISSN
0894-1491

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✦ Synopsis

A cDNA clone encoding a stimulatory G-protein a subunit (Gsa) was isolated from a cDNA library derived from cultured rat astrocytes. The nucleotide sequence of the cDNA indicated that it corresponds to the Gsa-2 form of Gsa mRNA, one of four Gsa mRNAs known to be derived by alternative splicing from the human Gsa gene. A ribonuclease protection assay using cRNA from this clone allowed distinction between the Gsa-l and Gsa-2 mRNAs, which encode the 52-kDa (Gs-L) forms of Gsa. Astrocytes express relatively high amounts of Gsa-l mRNA, much lower amounts of the Gsa-2 mRNA, and no detectable amounts of the mRNAs (Gsa-3 and Gsa-4) encoding the two 45-kDa forms of Gsa (Gp-S). Similar results were obtained with RNA samples isolated from whole brain. The 45-kDa form of Gsa protein was not detectable by immunoblot analysis of a membrane preparation from rat cerebral cortex (the source of the astrocyte cultures). These results indicate that the expression of Gsa forms in astrocytes is similar to that found in whole brain.

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