Stimulated indole alkaloid release from Catharanthus roseus immobilized cultures. Initial studies
β Scribed by B Jardin; R Tom; C Chavarie; D Rho; J Archambault
- Book ID
- 119131676
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- English
- Weight
- 927 KB
- Volume
- 21
- Category
- Article
- ISSN
- 0168-1656
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Catharanthus roseus hairy root cultures, genetically transformed with Agrobacterium rhizogenes, produce a wide variety of indole alkaloids. The effect of sucrose, phosphate, nitrate, and ammonia concentrations on growth and indole alkaloid production of C. roseus hairy root cultures were studied by
Cultures of C. roseus transgenic (''hairy'') root clones LBE-6-1 and LBE-4-2 were adapted with periodic daily illumination to investigate the effect of light on growth and nutrient utilization, and the accumulation of the indole alkaloids. Light-adapted roots appeared green and had radially thickene
Batch shake flask studies with Catharanthus roseus demonstrated that alkaloid production commenced only after growth had slowed or ceased. To obtain high alkaloid productivities for extended periods, a hormone-free production medium was used. To develop a readily scalable process, both immobilized a